Chloroplast Research in Arabidopsis: Methods and Protocols, Volume I - Methods in Molecular Biology 2011 edition

Marc Notes: Includes bibliographical references and index. Jacket Description/Back: Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable. The relatively recent adoption of "Arabidopsis thaliana" as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid. In "Chloroplast Research in Arabidopsis: Methods and Protocols," expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. "Volume I" explores topics such as genetics, cytology, "in vivo" analysis, gene expression, and protein accumulation, as well as protein transport, localization, and topology. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, "Chloroplast Research in Arabidopsis: Methods and Protocols" serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes."Table of Contents: Preface -- Contributors -- Part I. Genetics, Cytology, and In Vivo Analysis -- 1. Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis / Enrique LOpez-Juez, Alison Hills -- 2. Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy / Kevin Pyke -- 3. Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins / Stanislav Vitha, Katherine W. Osteryoung -- 4. Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts / Dong Wook Lee, Inhwan Hwang -- 5. Visualisation of Stromules on Arabidopsis Plastids / John C. Gray, James A. Sullivan, Christine A. Newell -- 6. Analysis of Chloroplast Movement and Relocation in Arabidopsis / Masamitsu Wada, Sam-Geun Kong -- 7. Studying Starch Content and Sedimentation of Amyloplast Statoliths in Arabidopsis Roots / John Stanga, Allison Strohm, Patrick H. Masson -- 8. Studying Arabidopsis Chloroplast Structural Organisation Using Transmission Electron Microscopy / Stefan Hyman, R. Paul Jarvis -- 9. Transplastomics in Arabidopsis: Progress Toward Developing an Efficient Method / Kerry Ann Lutz, Arun Azhagiri, Pal Maliga -- Part II. Gene Expression and Protein Accumulation -- 10. Isolation, Quantification, and Analysis of Chloroplast DNA / Beth A. Rowan, Arnold J. Bendich -- 11. Measurement of Transcription Rates in Arabidopsis Chloroplasts / Yan O. Zubo, Thomas BOrner, Karsten Liere -- 12. Studying the Structure and Processing of Chloroplast Transcripts / Alice Barkan -- 13. In Vitro RNA-Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts / Toshiharu Shikanai, Kenji Okuda -- 14. Studying Translation in Arabidopsis Chloroplasts / Paolo Pesaresi -- 15. Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts / Lars L. E. SjOgren, Adrian K. Clarke -- Part III. Protein Transport, Localization, and Topology -- 16. In Silico Methods for Identifying Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins / Sandra K. Tanz, Ian Small -- 17. Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays / Henrik Aronsson, R. Paul Jarvis -- 18. Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions / Hitoshi Inoue, Fei Wang, Takehito Inaba, Danny J. Schnell -- 19. Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays / Thomas Bals, Danja SchUnemann -- 20. Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction / Chiung-Chih Chu, Hsou-min Li -- 21. Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases / John Froehlich -- Index.