Cell Migration: Developmental Methods and Protocols - Methods in Molecular Biology 2nd ed. 2011 edition

Marc Notes: Includes bibliographical references and index. Table of Contents:1. Cell Migration: An Overview Miguel Vicente-Manzanares and Alan Rick Horwitz 2. Scratch-Wound Assay Giles Cory 3. HGF-Induced DU145 Cell Scatter Assay Sally T. Fram, Claire M. Wells, and Gareth E. Jones 4. Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time Sanjay Chaubey, Anne J. Ridley, and Claire M. Wells 5. Imaging Cells Within 3D Cell Derived Matrix Samantha J. King and Maddy Parsons 6. Live Cell Imaging of Neuronal Growth Cone Motility and Guidance In Vitro Daniel M. Suter 7. Boyden Chamber Marco Falasca, Claudio Raimondi, and Tania Maffucci 8. Transwell(r) Invasion Assays John Marshall 9. Imaging Podosome Dynamics and Matrix Degradation Taylor W. Starnes, Christa L. Cortesio, and Anna Huttenlocher 10. Endothelial Cell Migration Under Flow Beata Wojciak-Stothard 11. In Vitro Analysis of Chemotactic Leukocyte Migration in 3D Environments Michael Sixt 12. Quantification of Transendothelial Migration Using Three-Dimensional Confocal Microscopy Robert J. Cain, Barbara Borda d Agua, and Anne J. Ridley 13. Chemotaxis of Slow Migrating Mammalian Cells Analyzed by Video Microscopy Roman Zantl and Elias Horn 14. Live Cell Fluorescence Microscopy Techniques Shawn A. Galdeen and Alison J. North 15. Measuring Invasion in an Organotypic Model Veronika Jenei, Maria L. Nystrom, and Gareth J. Thomas 16. Analysis of Cell Migration Using "Caenorhabditis elegans" as a Model System Ming-Ching Wong, Maria Martynovsky, and Jean E. Schwarzbauer 17. "Drosophila" Hemocyte Migration: An In Vivo Assay for Directional Cell Migration Carolina G. A. Moreira, Jennifer C. Regan, Anna Zaidman-Remy, Antonio Jacinto, and Soren Prag 18. Measuring Inflammatory Cell Migration in the Zebrafish Philip M. Elks, Catherine A. Loynes, and Stephen A. Renshaw 19. Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement Mohit Prasad, Xiaobo Wang, Li He, and Denise J. Montell 20. Assessment of Development and Chemotaxis in "Dictyostelium discoideum" Mutants Yulia Artemenko, Kristen F. Swaney, and Peter N. Devreotes 21. Simple Experimental and Spontaneous Metastasis Assays in Mice Gary M. Box and Suzanne A. Eccles 22. Two-Photon Intravital Multicolour Imaging to Study Metastatic Behaviour of Cancer Cells In Vivo Sylvia E. Le Devedec, Wies van Roosmalen, Chantal Pont, Reshma Lalai, Hans de Bont, and Bob van de Water 23. Measuring Angiogenesis in Mice Bernardo Tavora and Kairbaan Hodivala-Dilke 24. Time-Lapse Imaging of Chick Cardiac Precursor Cells Junfang Song, Qiaoyun Yue, and Andrea Munsterberg 25. Characterizing System Performance in Total Internal Reflection Fluorescence Microscopy Juliane P. Schwarz, Ireen Konig, and Kurt I. Anderson 26. Fluorescence Recovery After Photobleaching Alex Carisey, Matthew Stroud, Ricky Tsang, and Christoph Ballestrem 27. Measuring FRET Using Time-Resolved FLIM Penny E. Morton and Maddy Parsons 28. Cell Migration in Confinement: A Micro-Channel Based Assay Melina L. Heuze, Olivier Collin, Emmanuel Terriac, Ana-Maria Lennon-Dumenil, and Matthieu Piel 29. Functional Screening with a Live Cell Imaging Based Random Cell Migration Assay Wies van Roosmalen, Sylvia E. Le Devedec, Sandra Zovko, Hans de Bont, and Bob van de Water 30. Beads on the Run: Beads as Alternative Tools for Chemotaxis Assays Eric Theveneau and Roberto Mayor"Jacket Description/Back: Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study. The aim of "Cell Migration: Developmental Methods and Protocols"," Second Edition" is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances. Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D. It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful "Methods in Molecular Biology " series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, "Cell Migration: Developmental Methods and Protocols, Second Edition" provides a comprehensive catalogue of techniques for the study of cell migration that can be used as a useful reference source for any researcher who wishes to explore this significant area of cell biology."